A disposable Laccase–Tyrosinase based biosensor for amperometric detection of phenolic compounds in must and wine
نویسندگان
چکیده
An amperometric biosensor for the detection of polyphenols in wine has been developed immobilizing the two enzymes Tyrosinase and Laccase on graphite screen printed electrodes modified with ferrocene. Different immobilization procedures have been carried out, the sensor operational parameters have been optimized, determining the best conditions and the analytical method for the analysis of samples. The creen printed electrodes olyphenols ines ulphur dioxide biosensor has been then testedwith real samples, usingwines andmusts supplied by Astra, experimental winery, in Imola (Italy). Thebiosensorgavegood resultswhenemployed forwineanalysis, showingagood agreement with the spectrophotometric data obtained with the Folin-Ciocalteu test, the official method for polyphenols’ analysis in wine. On the other hand, the measurements on musts and wines recently bottled, were seriously affected by the presence of an high level of free sulphur dioxide. SO2 is the likely responsible for enzyme activity inhibition on the sensor. Further studies are currently proceeding to find ditio out the most suitable con
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